Methods Electrophysiology Records

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Record
  ID
  ID Literature
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  Solution
  Recording Method
  Stimulation Method
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  Reference Text
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  Gen Comments
Literature.ID Ref.
Methods Electrophysiology Animals.ID Methods Electrophysiology Ref.
Methods Electrophysiology CompConc.ID Methods Electrophysiology Ref.
Methods Electrophysiology SliceOrient.ID Methods Electrophysiology Ref.
Neurons Connectivity.ID Methods Electrophysiology Ref.
Neurons FiringProperties.ID Methods Electrophysiology Ref.
Neurons IonicConductances.ID Methods Electrophysiology Ref.
Neurons IonicCurrents.ID Methods Electrophysiology Ref.
Neurons Morphology.ID Methods Electrophysiology Ref.
Neurons SynapticCurrents.ID Methods Electrophysiology Ref.
-2086957548 -2086957548 KS00 Acute Slice Na/K Block Nucleated outside-out patch Voltage steps Rat 20-22 pp.622-623 - "In addition to TEA and 4-AP, several selective K+ channel toxins were tested.
MCD-P (1 µM, n=4), BDS-I (100 nM, n=3), TsTx K-alpha (200 nM, n=3), MgTx (300
nM, n=3), StTx (300 nM, n=3) and AgTx (60 nM, n=3) had no effect on either the
fast or the slow K+ currents. DTX-I (250 mM, n=4) reduced the amplitude of the
slow K+ current to 84 +- 7% of the control values without affecting its
activation or inactivation curves (data not shown). DTX-I did not affect the
fast K+ current."p.630 		JDJ 0 KS00 -2086957548 -2086957548 -2086957548       1006790213    
-1892354029 -1892354029 TBGW03 Acute Slice Ca buffer + Glu block Sharp electrode recordings Somatic current injection Rat 35 p.912 - - JDJ 0 TBGW03   -1892354029     1064194824        
-1756532732 -1756532732 BSC94 Dissociated Neurons K Block/Low Na/Papain Whole-cell Vclamp Voltage steps Rat - pp.2562-2563 - Used only for Ina recordings with Papain removed inactivation. JDJ 0 BSC94 -1756532732 -1756532732         406866101    
-1723982012 -1723982012 OSC01a Acute Slice Na Block Whole-cell V-rec/[Ca2+]i-imaging Focal glutamate iontophoresis Rat 29-32 pp.504-505 - Cells loaded with Fluo-4 and Lucifer yellow for [Ca2+]i imaging and cell
visualization. 		JDJ 0 OSC01a -1723982012 -1723982012 -1723982012            
-1671068009 -1671068009 TBGW03 Comparmental model Standard - Somatic current injection - - pp.910-912 Fig. 1 - JDJ 0 TBGW03         1064194824 -558364987      
-1665514842 -1665514842 TBGW03 Comparmental model KC block - Somatic current injection - - pp.910-912 Fig. 1 - JDJ 0 TBGW03         1064194824        
-1662948559 -1662948559 MFA98 Dissociated Neurons K/Ca Block Whole-cell Vclamp Voltage ramps Rat 21-24 pp.106-107 - TTX locally applied. JDJ 0 MFA98 -1662948559 -1662948559 -1662948559       989418859    
-1633605921 -1633605921 OSC01b Acute Slice Na Block Whole-cell V-rec Dual focal glutamate iontophoresis (+ hyperpolarizing current inj.) Rat 29-32 pp.515 - Cells loaded with Lucifer yellow for cell visualization. JDJ 0 OSC01b -1633605921 -1633605921 -1633605921   1940208406        
-1610535617 -1610535617 KS00 Acute Slice Na Block Nucleated outside-out patch Voltage steps Rat 20-22 pp.622-623 - Cells visualized using IR-DIC. Influence of Ca2+ currents (and thus Ca2+
activated K+) were shown to be neglible (p.623). 		JDJ 0 KS00 -1610535617 -1610535617 -1610535617       1006790213    
-1460621992 -1460621992 TBGW03 Comparmental model Ca buffer - Somatic current injection - - pp.910-912 Fig. 1 - JDJ 0 TBGW03         1064194824        
-1403035255 -1403035255 OSC01b Acute Slice Standard Whole-cell V-rec Distal apical dendrite: Focal glutamate iontophoresis Rat 29-32 pp.515 - Cells loaded with Lucifer yellow for cell visualization. JDJ 0 OSC01b -1403035255   -1403035255   1940208406        
-1357204228 -1357204228 MFA98 Dissociated Neurons Na/K Block Whole-cell Vclamp Voltage steps Rat 21-24 pp-106-107 - - JDJ 0 MFA98 -1357204228 -1357204228 -1357204228       989418859    
-1322394210 -1322394210 MFA98 Dissociated Neurons K/Ca Block/Low Na Whole-cell Vclamp Voltage steps Rat 21-24 pp.106-107 - TTX applied locally. JDJ 0 MFA98 -1322394210 -1322394210 -1322394210       989418859    
-1195798297 -1195798297 BGC00 Acute Slice Standard Whole-cell Iclamp Extracellular tetanic stim. Rat 32 pp.909-910 - - JDJ 0 BGC00 -1195798297 -1195798297 -1195798297            
-1143564358 -1143564358 LESF00 Acute Slice Standard Whole-cell V-rec Loose patch Rat 20-23 pp.5300-5303 Fig.1 - JDJ 0 LESF00 -1143564358 -1143564358 -1143564358 -1926191548       -2088194568  
-1135746851 -1135746851 KHP96a Dissociated Neurons Ca Block Inside-out patch V-clamp/ramp Rat 21-22 p.189-190 Fig.1 "These low concentrations of enzymes were used because preliminary experiments
showed that cencentrations > 0.5 mg/ml markedly affected the voltage
dependence and Ca2+ sensitivity of BK channels in young but not older
animals."p.189 (referring to dissociation method). 		JDJ 0 KHP96a -1135746851 -1135746851 -1135746851     -1208544038      
-1067964602 -1067964602 TBGW03 Compartmental model Standard - Dendritic current injection - - pp.910-912 Fig. 1 - JDJ 0 TBGW03         1064193356        
-1051416814 -1051416814 OSC01a Acute Slice Ca Block Whole-cell V-rec/[Ca2+]i-imaging Focal glutamate iontophoresis Rat 29-32 pp.504-505 - Cells loaded with Fluo-4 and Lucifer yellow for [Ca2+]i imaging and cell
visualization. 		JDJ 0 OSC01a -1051416814 -1051416814 -1051416814       -342234224    
-1020917390 -1020917390 ASC93a Dissociated Neurons K/Ca Block Whole-cell patch Depolarizing voltage steps/ramps Rat 21-24 pp.290-291 - - JDJ 0 ASC93a -1020917390 -1020917390         481039627    
-979866364 -979866364 SC95 Acute Slice Na/Ih Block Whole-cell Vclamp Glutamate iotophoresis Rat 31-34 p.2220 Fig. 1 - JDJ 0 SC95   -979866364              
-862865877 -862865877 HHP91 Dissociated Neurons Na/Ca Block Whole-cell Vclamp Command potentials Rat 22-24 pp.48-50 Table 1 - JDJ 0 HHP91 -862865877 -862865877         216257617    
-850117393 -850117393 BSC94 Dissociated Neurons K Block/Low Na Whole-cell Vclamp Voltage steps Rat - pp.2562-2563 - Used only for Ina recordings. JDJ 0 BSC94 -850117393 -850117393         406866101    
-847634651 -847634651 SSSS97 Acute Slice AMPA/NMDA Block Whole-cell V-rec Synaptic input via laterally separated electrode Rat 35+-0.5 p.606 - "High speed [Ca2+]i fluorescence measurements were performed using the
calcium-sensitive fluorescent dye Calcium Green-1 (CG-1; Kd, 315 nM; Molecular
Probes) and a frame transfer cooled CCD camera (ATC-5m Photometrics, Tucson,
AZ, USA)."p.606 		JDJ 0 SSSS97 -847634651 -847634651     -2140593619   1000126375   776753756
-795178879 -795178879 TBGW03 Acute Slice NaP enhanced + Glu block Sharp electrode recordings Somatic current injection Rat 35 p.912 - - JDJ 0 TBGW03 -795178879 -795178879     1064193356        
-754473072 -754473072 HHP91 Dissociated Neurons Na/K Block Whole-cell Vclamp Command potentials Rat 22-24 pp.48-50 Table 1 - JDJ 0 HHP91 -754473072 -754473072         216257617    
-625163851 -625163851 SC95 Acute Slice Na/Ih/NMDAR Block Whole-cell Vclamp Glutamate iotophoresis Rat 31-34 p.2220 Fig. 1 - JDJ 0 SC95 -625163851 -625163851              
-603030912 -603030912 TBGW03 Acute Slice NaP block + Glu block Sharp electrode recordings Somatic current injection Rat 35 p.912 - - JDJ 0 TBGW03 -603030912 -603030912     1064194824        
-586292050 -586292050 TBGW03 Acute Slice KC block + Glu block Sharp electrode recordings Somatic current injection Rat 35 p.912 - - JDJ 0 TBGW03 -586292050 -586292050     1064194824        
-399777519 -399777519 FGPSWA95 Acute Slice Na/K/Ca Block Iclamp Current pulses Rat 35 p.128, - - JDJ 0 FGPSWA95 -399777519 -399777519 -399777519   996756505        
-315767312 -315767312 MFA98 Acute Slice Standard+Kynurenic acid Whole-cell Iclamp Current pulse Rat 35 pp.106-107 - - JDJ 0 MFA98 -315767312 -315767312 -315767312   989418859        
-253258512 -253258512 MLSC97 Acute Slice Standard Intracellular V-clamp Depolarizing current pulse Rat 31 pp.1188-1189 - - JDJ 0 MLSC97 -253258512       -1546107746        
-232296371 -232296371 HHP89 Dissociated Neurons K/Ca Block Cell attached patch Differential microperfusion/voltage steps Rat 22-24 pp.2473-2474 Fig.1 - JDJ 0 HHP89 -232296371 -232296371 -232296371       -9806123    
-231029841 -231029841 LF95a Dissociated Neurons Na/K/Ca Block Whole-cell patch Voltage steps/ramps Rat room pp.1431-1432 - Ba2+ used as charge carrier in some measurements. Ca2+ channel blocker
concentrations determined as saturating concentration (ie. Higher dose did not
produce higher larger effect). 		JDJ 0 LF95a -231029841 -231029841 -231029841       999088458    
-192623055 -192623055 MLSC97 Acute Slice K/Ca Block Intracellular I-clamp/Optical Depolarizing current pulse Rat 31 pp.1188-1189 - - JDJ 0 MLSC97 -192623055 -192623055         987766595    
-135019817 -135019817 KHP96a Dissociated Neurons Low Ca Inside-out patch V-clamp/ramp, varying inside Ca-perfusate Rat 21-22 p.189-190 Fig.1 "These low concentrations of enzymes were used because preliminary experiments
showed that cencentrations > 0.5 mg/ml markedly affected the voltage
dependence and Ca2+ sensitivity of BK channels in young but not older
animals."p.189 (referring to dissociation method). 		JDJ 0 KHP96a -135019817 -135019817 -135019817     -2090324328      
-62810572 -62810572 SSSS97 Acute Slice Na Block Whole-cell V-rec Synaptic input via laterally separated electrode/current injection Rat 35+-0.5 p.606 - "High speed [Ca2+]i fluorescence measurements were performed using the
calcium-sensitive fluorescent dye Calcium Green-1 (CG-1; Kd, 315 nM; Molecular
Probes) and a frame transfer cooled CCD camera (ATC-5m Photometrics, Tucson,
AZ, USA)."p.606 		JDJ 0 SSSS97 -62810572 -62810572         1000126375    
-20718951 -20718951 HHP88 Dissociated Neurons Na Block Whole-cell patch Voltage step-depolarizations Rat 23 p.783-784, reducing developing large Na-currents - TEA in pipette solution (10mM) JDJ 0 HHP88 -20718951 -20718951 -20718951       35614620 35614620  
39353653 39353653 ZC99 In Vivo Standard Blind whole-cell Whisker displacement Rat 37.2+-0.2 p-1172 - - JDJ 0 ZC99 39353653       -225112611       -225112611
40296339 40296339 OSC01b Acute Slice Na Block Whole-cell V-rec Focal glutamate iontophoresis Rat 29-32 pp.515 - Cells loaded with Lucifer yellow for cell visualization. JDJ 0 OSC01b 40296339 40296339 40296339   1940208406        
83751301 83751301 KS00 Acute Slice Na Block Cell attached patch Voltage steps Rat 20-22 pp.622-623 - - JDJ 0 KS00 83751301 83751301 83751301     1006790213      
180206585 180206585 OSC01b Acute Slice Ca Block Whole-cell V-rec Focal glutamate iontophoresis Rat 29-32 pp.515 - Cells loaded with Lucifer yellow for cell visualization. JDJ 0 OSC01b 180206585 180206585 180206585   1940208406        
189932712 189932712 OSC01b Acute Slice Standard Whole-cell V-rec Oblique apical dendite:Focal glutamate iontophoresis+soma hyperpolarization Rat 29-32 pp.515 - Cells loaded with Lucifer yellow for cell visualization. JDJ 0 OSC01b 189932712   189932712   1940208406        
315966505 315966505 BSC93 Dissociated Neurons Na/K/Ca Block Whole-cell Vclamp Voltage pulses Rat 20-22 pp.1531-1532 - Kynurenic acid (2mM) also added to block possible activation of postsynaptic
excitatory amino acid receptors (p.1531). 		JDJ 0 BSC93 315966505 315966505 315966505       1004375359    
515071704 515071704 OSC01b Acute Slice NMDA Block Whole-cell V-rec Focal glutamate iontophoresis Rat 29-32 pp.515 - Cells loaded with Lucifer yellow for cell visualization. JDJ 0 OSC01b 515071704 515071704 515071704   1940208406        
655797886 655797886 WS00 Acute Slice Na/K Block Cell attached patch Simulated EPSPs Rat 20-24/34-35 pp.3177-78 - - JDJ 0 WS00 655797886 655797886 655797886           -381558745
663618204 663618204 BGC00 Acute Slice Standard Whole-cell Iclamp ACPD/DHPG Rat 32 pp.909-910 - - JDJ 0 BGC00 663618204   663618204   976538574       -990651240
732686535 732686535 MFA98 Dissociated Neurons Na Block Whole-cell Vclamp Voltage steps Rat 21-24 pp.106-107 - TTX solution applied locally. JDJ 0 MFA98 732686535 732686535 732686535       989418859    
842933658 842933658 TBGW03 Compartmental model NaP/Ca block - Somatic current injection - - pp.910-912 Fig. 1 - JDJ 0 TBGW03         1064194824        
974208830 974208830 HHP88 Dissociated Neurons Standard Cell attached patch Depolarizing voltage step Rat 23 p.783 - 4-aminopyridine in recording pipette JDJ 0 HHP88 974208830 974208830 974208830       35614620    
974817158 974817158 SSC90 Dissociated Neurons Na/K Block Whole-cell Vclamp Depolarizing voltage steps Rat 20-23 p.175-176 - - JDJ 0 SSC90 974817158 974817158 974817158       -806603121    
974903425 974903425 SSC90 Dissociated Neurons Ca Block Whole-cell Vclamp Depolarizing voltage step Rat 23 pp.176-177 Tab. 1, Fig.2 Still contains TEA and TTX as in first record (974817158). JDJ 0 SSC90 974903425 974903425 974903425       -806603121    
975923916 975923916 ZC99 In Vivo Standard Blind whole-cell Current injection Rat 37.2+-0.2 p-1172 - - JDJ 0 ZC99         -225112611     -1387839592  
976026094 976026094 KHP96a Dissociated Neurons Ca Block Outside-out patch Voltage clamp Rat 21-22 pp.189-190 Fig.1 "These low concentrations of enzymes were used because preliminary experiments
showed that cencentrations > 0.5 mg/ml markedly affected the voltage
dependence and Ca2+ sensitivity of BK channels in young but not older
animals."p.189 (referring to dissociation method). 		JDJ 0 KHP96a 976026094 976026094 976026094     -1208544038      
976116286 976116286 BGC00 Acute Slice Standard Whole-cell Iclamp I-pulse Rat 32 pp.909-910 - - JDJ 0 BGC00 976116286   976116286 -990651240 157159084        
976202835 976202835 BGC00 Acute Slice Standard Whole-cell Iclamp Muscarine Rat 32 pp.909-910 - - JDJ 0 BGC00 976202835   976202835           -990651240
986573713 986573713 WS00 Acute Slice Na/K Block Cell attached patch Voltage steps Rat 20-24/34-35 pp.3177-78 - - JDJ 0 WS00 986573713 986573713 986573713   -381558745   -781327707    
987614460 987614460 HHP89 Dissociated Neurons K/Ca Block Whole-cell Vclamp Differential microperfusion/voltage steps Rat 22-24 pp.2473-2474 Fig.1 - JDJ 0 HHP89 987614460 987614460 987614460       -9806123    
988275445 988275445 HHP91 Dissociated Neurons Standard Whole-cell Iclamp Current injection Rat 22-24 pp.48-50 Table 1 - JDJ 0 HHP91 988275445       216257617     216257617  
988800474 988800474 FG96 Acute Slice K/Ca Block Whole-cell patch Current pulse/voltage ramps Mouse 32+-0.5 p.2125 - - JDJ 0 FG96 988800474 988800474 988800474       -1725086893    
988981771 988981771 MFA98 Acute Slice Standard Whole-cell Iclamp Current pulse Rat 35 pp.106-107 - - JDJ 0 MFA98 988981771   988981771   989418859        
996239748 996239748 BSSC94 Dissociated Neurons Na/K/Ca Block Whole-cell Vclamp Voltage pulses Rat - p.198 - No temperature given. Different sequences/combinations of the calcium blockers.
Omega-Aga-IVA, omega-CgTX and nifedipine applied by puffer-pipette. Cd-ions in
bath.

"It should be realized that the isolated neurones used in these experiments
have lost almost all of their dendritic tree during dissociation. Thus, the
proportion of calcium channel subtypes making up the HVA current may be quite
different in intact cortical neurones."p.204. 		JDJ 0 BSSC94 996239748 996239748         -1041488843    
996488643 996488643 FGPSWA95 Acute Slice Standard Iclamp Current pulses Rat 35 p.128 - - JDJ 0 FGPSWA95 996488643   996488643   996756505     996756505  
996581200 996581200 FGPSWA95 Acute Slice Na/K Block Iclamp Current pulses Rat 35 p.128, 133 - - JDJ 0 FGPSWA95 996581200 996581200 996581200   996756505        
998918972 998918972 KHP96b Dissociated Neurons K+varying [Ca2+] Inside-out patch Holding & ramp potentials Rat 21-22 p.4194 - Single channel recordings. JDJ 0 KHP96b 998918972         998919415      
998925852 998925852 KLLB94a Acute Slice Standard Whole-cell V-rec Current pulse Rat 35 p.461 - Also description of tracer injections. JDJ 0 KLLB94a 998925852   998925852   998993756        
999005478 999005478 LF95a Dissociated Neurons Na/K Block Whole-cell patch Voltage steps/ramps Rat room pp.1431-1432 - Ba2+ used as charge carrier in some measurements. JDJ 0 LF95a 999005478 999005478 999005478       999088458    
999097811 999097811 FSPRA98 Acute Slice Standard Current clamp Current pulses Rat 35 pp.1013-1014 - - JDJ 0 FSPRA98 999097811   999097811