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1006790213
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Neurons IonicCurrents
68 records
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investigate the kinetics of this current. The kinetics were quantified by
fitting the fast K+ current to a Hodgkin-Huxley model. To increase the tail
current amplitude the K+ concentration in the ACSF was increased to 65 mM.
This change in K+ did not change the activation or deactivation kinetics of
the fast K+ current (n = 7, data not shown). A Hodgkin-Huxley model with four
activation gates (Fig. 4B) best desribed the activation and deactivation time
course of the fast K+ current. The time constants, extracted from this
analysis, ranged from 0.31 +- 0.03 ms at +80 mV (n=5) and displayed a
bell-shaped dependence on voltage (Fig. 4C)."pp.625-626
"The inactivation time constant of the fast K+ current was measured by
mono-exponential fits to the decay phase of the current (Fig. 5A). Above -20
mV the inactivation time constant was not dependent on voltage with a mean
value of 8.0 +- 0.3 ms (n=5)."p.626
"The rate of recovery from inactivation ranged from 12.6 +- 1.5 ms (n = 5) at
-110 mV to 64 +- 11 ms (n = 4) at -70 mV."p.626
"4-AP (3 mM) reduced the maximal amplitude of both fast and slow K+ currents
as recorded following a voltage step to +80 mV (Fig. 6A). ... The
concentration of 4-AP yielding half the current observed under control
conditions was 4.2 +- 0.5 mM (Fig. 6B)."p.629
