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1004375359
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Neurons FiringProperties
65 records
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hyperpolarizing voltage steps of 10 ms duration and 10-30 mV amplitude from a
holding potential of -60 mV were employed to calculate input resistance and to
estimate cell surface area (Fig. 1B; see methods). The decay of the capacitive
current transient was fitted best by two exponential functions. The fastest
time constant (6-65 µs) was likely due to the charging og the pipette tip (and
may have been lengthened artificially by low-pass filtering of these records).
The slower time constant (0.40-2.38 ms) was likely due to the capacitance of
the membrane because the surface area derived from this time constant
correlated with other measures of surface area (see METHODS). Passive
properties measured in 22 cells were as follows: input resistance, 3.5 +- 0.4
GOhm (mean +- SE); specific membrane resistance, 51.9 +- 6.8 KOhm-cm^2; cell
capacitance, 17.5 +- 1.2 pF (range: 7.0-29.8 pF)."p.1532
17.5+-1.2pF (range 7.0-29.8 pF). Capacitance related to truncated cells
(acutely dissociated neurons).
