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KHP96b
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Literature
38 records
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channels) were investigaed in neocortical infragranular pyramidal neurons by
the use of inside-out patch recordings. Neurons were acutely isolated from
slices of newborn to 28-day-old rats (P0-P28) by using minimal protease
exposure followed by trituration with a vibrating glass probe. Two types of BK
channels, slow-gating and fast-gating, were observed in immature neurons
(P0-P5), whereas only slow-gating BK were found in more mature neurons.
Fast-gating BK channels differed in conductance, voltage dependence, and
kinetics from the slow-gating ones. 2. The properties of fast-gating channels
included a conductance of 145 +/- 12.9 (SE) pS; frequent openings with short
mean open times that were relatively voltage-independent, mean closed times
that showed a voltage-dependent increase, a voltage-dependent decrease in open
probability (Po). The properties of slow-gating channels contrasted with those
of the fast-gating ones, in that the former had a conductance of 181 +/- 3.9
pS, longer mean open times that showed a voltage-dependent increase, mean
closed times that showed a marked voltage-dependent decrease, a
voltage-dependent increase in Po, and slight inward rectification. The
significance of these developmental variations in channel properties is
discussed.
neurons) entered.
